15361

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15361

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Bleomycin sulfate from Streptomyces verticillus

BioChemika, for fluorescence, mixture of bleomycin sulfate salts, lyophilized, powder, white

Synonym:Blenoxane, Bleo, Blexane
CAS Number:9041-93-4
EC Number:232-925-2
MDL number:MFCD00070310
PubChem Substance ID:24849358

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References

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Description

ApplicationRecommended for use as a selection agent at 10-100 μg/ml.
Other NotesDNA degradation1,2; Action mechanism, minireview3
Biochem/physiol ActionsAn antineoplastic antibiotic isolated from Streptomyces verticillus. Binds to DNA, inhibits DNA synthesis and causes DNA scissions at specific base sequences. Needs to bind oxygen and a metal ion such as copper or iron to cleave DNA. Highly selective cleavage of RNA. Inducer and regulator of apoptosis in a variety of cells. Inhibits tumor angiogenesis.

Properties

gradefor fluorescence
product lineBioChemika
formpowder
qualitylyophilized
color white
solubilityH2O: 20 mg/mL, clear, colorless
cation tracesCu: ≤1000 mg/kg
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type P2 (EN 143) respirator cartridges
Hazard CodesT
Risk Statements46-40
Safety Statements53-36/37-45
WGK Germany3
RTECSEC5991990

References

Cited Reference1. V. Murray et al. J. Biol. Chem. 263, 12854, (1988) Abstract
 2. R.P. Hertzberg et al. Biochemistry 27, 3164, (1988) Abstract
 3. R.M. Burger et al. Life Sci. 28, 715, (1981) Abstract
referenceHuttenhofer, A., et al., Cleavage of tRNA by Fe(II)-bleomycin. J. Biol. Chem. 267, 24471-24475, (1992)
 Holmes, C.E, et al., Fe bleomycin as a probe of RNA conformation. Nucleic Acids Res. 24, 3399-3406, (1996)
 Maggliozzo, R.S, et al., Transfer RNA is cleaved by activated bleomycin. Mol. Pharmacol. 35, 428-432, (1989)
 Morgan, M.A. and Hecht, S.M., Iron (II) bleomycin-mediated degradation of a DNA-RNA heteroduplex. Biochemistry 33, 10286-10293, (1994)
 Hamilton, R.F., et al., Bleomycin induces apoptosis in human alveolar macrophages. Am. J. Physiol. 26, L318-L325, (1995)
 Mir, L.M., et al., Bleomycin: revival of an old drug. Gen. Pharmacol. 27, 745-748, (1996)
 Sam, J.W., et al., Sequence-specific changes in the metal site of ferric bleomycin induced by the binding of DNA. J. Biol. Chem. 273, 16090-16097, (1998)
 Holmes, C.E, et al., On the chemistry of RNA degradation by Fe bleomycin. Bioorg. Med. Chem. 5, 1235-1248, (1997)
 Vernole, P, et al., Induction of apoptosis by bleomycin in resting and cycling human lymphocytes. Mutagenesis 13, 209-215, (1998)
 Christensen, J.G., et al., Regulation of apoptosis in mouse hepatocytes and alteration of apoptosis by nongenotoxic carcinogens. Cell. Growth Differ. 9, 815-825, (1998)
 Araki, T, et al., Changes in c-Jun but not Bcl-2 family proteins in p53-dependent apoptosis of mouse cerebellar granule neurons induced by DNA damaging agent bleomycin. Brain Res. 794, 239-247, (1998)
 Kross, J, et al., Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin and tallysomycin. Biochemistry 21, 4310-4318, (1982)
 Kross, J, et al., Structural basis for the deoxyribonucleic acid affinity of bleomycins. Biochemistry 21, 3711-3721, (1982)
 Carter, B.J, et al., Site-specific cleavage of RNA by Fe(II) bleomycin. Proc. Natl. Acad. Sci. U. S. A. 87, 9373-9377, (1990)
 Schirner, M., et al., Antiangiogenic chemotherapeutic agents: characterization in comparison to their tumor growth inhibition in human renal cell carcinoma models. Clin. Cancer Res. 4, 1331-1336, (1998)
MerckMerck 13,1311